I've used both drives together on a laptop with no issues. There is one thing to keep in mind, XP & below has problems installing on a drive over 137GB (SATA drives). As to why, I can't answer with certainty, because I've read and heard numerous things in that regard. I would certainly like to find an straight answer to that issue.
A desktop cools much better than a laptop would, so unless there's a problem with the drive, it should run fine. As far as 24/7 goes, this old laptop has run that way for nearly two months now, and still running fine. With all of the extra cooling that the drive would be getting in the desktop, and you being able to keep the dust out better, it should last longer than it would in the laptop. You may want to monitor it for a few days, just to make sure.
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